Synthesis , Physicochemical Identification With Antimicrobial And Anti-inflammatory Evaluation of Some New Metal Complexes Derived From Enprofylline

Abstract

     This study outlines the synthesis of the ligand [(4-((2,6-dioxo-3-propyl-2,3,4,5,6,7-hexahydro-1H-purin-8-yl) BAE] through a diazo coupling reaction between enprofylline and 4-amino-2-chlorobenzaldehyde. This ligand was then complexed with Co(II), Cu(II), Pd(II), Ag(I) and Pt(IV). The molecular structures of the resulting complexes were characterized using spectral including thermal and physicochemical techniques. The complexes exhibit a (1:2)(M:L) stoichiometry .The FTIR study corroborated that BAE ligand acts as neutral N,N-bidentate and chelated with selected metal ions  via(N-9) atom of imine group in imidazole and nitrogen atom of  azo group and they yield tetrahedral geometry for Ag-BAE, square planar for Pd-BAE and octahedral for the remained  complexes. All synthesized compounds demonstrated high thermogravimetric analysis (TGA) studies. Additionally, the calculated stability constant and ΔG of complexes confirmed the thermodynamic stability and spontaneous reaction . The BAE ligand and its complexes exhibit good antibacterial activity against two types of selected bacteria, when compared with amoxicillin drug as standard.  The antioxidant ability of Cu-BAE  and Pt-BAE complexes were examined by utilizing reductive activity and DPPH radical scavenging with Trolox and ascorbic acid as standard respectively ,the data indicate significant antioxidant activity. The(Pd-BAE)complex exhibited strong anti-inflammatory in wound healing assessments, showing significant effectiveness in treating burns and wounds ,which is took(9) days to heal, while the silver sulfadiazine drug as standard has (16) days and without treatment(18) days .Finally, the cytotoxic effectiveness of (Co-BAE) complex against the  colon cancer cell line CaCo₂ was investigated using MTT assay, revealing potential as an anticancer agent with IC50 109.1mg ml^-1 when compared with the normal cell line (WRL68)