Exploring the Potential Impact of L.acidophilus on Epidermal Growth Factor and Trefoil Factor (TFF)-2 in Alloxan-Induced Diabetic Rats.

Ali R  Mulakhudair; Zahraa Reasan Kareem  Shati; Ali  Al-Samydai; Amal Akour

doi:10.24996/ijs.2026.67.11.%g

Authors

Ali R Mulakhudair Department of Food Health and Nutrition, College of Food Science, Al-Qasim Green University, Babylon, 51013, Iraq https://orcid.org/0000-0001-8346-0288
Zahraa Reasan Kareem Shati Department of Food Health and Nutrition, College of Food Science, Al-Qasim Green University, Babylon, 51013, Iraq
Ali Al-Samydai Pharmacological and Diagnostic Research Centre, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, Jordan.
Amal Akour 3Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, P.O. Box 15551, United Arab Emirates./ Department of Biopharmaceutics and Clinical Pharmacy, The School of Pharmacy, University of Jordan, Amman, Jordan https://orcid.org/0000-0002-2789-8514

DOI:

https://doi.org/10.24996/ijs.2026.67.11.%25g

Keywords:

GI microbiome, Lactobacillus acidophilus, Dysbiosis, GI health, Probiotics

Abstract

Background: Probiotics have been linked to promoting healing processes in diabetic patients by modulating biomarkers such as epidermal growth factor (EGF) and trefoil factors (TFFs). Objective: The potential influence of probiotic supplementation on levels of Epidermal Growth Factor (EGF) and Trefoil Factor-2 (TFF-2) in alloxan-induced diabetic rats, and its implications for managing diabetes-related cellular changes, were examined in this study. Methods: Lactobacillus acidophilus was orally administered to diabetic rats weighing 140-160 grams, and its effects on biochemical and physiological indicators were assessed. The levels of blood cholesterol, liver and kidney function markers, and glucose were consistently monitored in the animal groups studied. Results: L. acidophilus had a significant positive impact on the tested parameters, resulting in a marked improvement in the health of the treated animals compared to the control group. The findings from this study highlight the importance of administering probiotics in diabetic animals. Future rigorous clinical studies should be conducted to evaluate their use as adjunctive therapy in patients with diabetes.